First Edition. The book will be useful to master s, bachelor s and doctoral students in biological sciences. It covers most of the practicals conducted at the master s level in biological sciences. The book contains 20 chapters describing practical protocols. The book starts from the chapters Colorimetry and Spectrophotometry where theoretical parts of these techniques are described. The book has a chapter that describes all the techniques used in plant tissue culture. The most important and salient feature of the book is that principles of the practical exercises as well as detailed protocols are described. Besides, the book covers principles and theoretical parts of many modern techniques, namely chromatography, electrophoresis, UV-VIS spectrophotometry, centrifugation, PCR and plant tissue culture.