Our knowledge of cell and tissue cultures has been developing with increasing knowledge, specialty in bio-transformation, forestry, genetic engineering, morphogenesis, somatic hybridization, maintaining pathogen free plants and rapid coplonal propagation, totipotency, differentiation, cell division, cell nutrition, metabolism, radion biology, cell preservation etc. It is now possible to cultivate cells in quantity, or as clones from single cells; to grow whole plant form isolated meristems and to induce and to induce callus or even single cell to develop in to complete plant either by organogenesis or directly by embrvogenesis in vitro. The production of pure hanploid plants through tissue culture from anthers or isolated microspores and of protoplasts from higher plants cells has served as the basis tools for genetic engineering and somatic hybridization. Tissue culture technique helps to propagate plants or economic importance such as orchids and other ornamental plants enlarge numbers buy their meristem culture of by either in vitro methods. This provides from vimsfree plantlets. Propagation of valuable economic plants though tissue culture basedon the principle to totipotency (every cell within the plant has the potential to give rise a whole plant). In plant breeding, ovary and ovule culture as well as in vitro pollination have been employed to overcome morphological and physiological sterility and incompatibility.